PE Mouse Anti-Human CD132

BD Pharmingen™ PE Mouse Anti-Human CD132

Name: PE Mouse Anti-Human CD132
Brand: BD Pharmingen™
SKU: 561699

Clone AG184

(RUO)

Flow cytometric analysis of CD132 expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human CD132 (Cat. No. 555900/561699; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.

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Product Details

Brand: BD Pharmingen™

Alternative Name: IL2RG; IL-2RG; IL-2Rγ; Common gamma chain; γc; CIDX; SCIDX; SCIDX1; IMD4

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Workshop Number: VI C-100

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Solution 10X Concentrate CE_IVD

Size 100 mL Cat No. 349202

Lysing Buffer RUO

Size 100 mL Cat No. 555899

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

PE Mouse Anti-Human CD132 RUO

Size 0.2 mg Cat No. 555900

561699 Rev. 2

Antibody Details

AG184

The AG184 monoclonal antibody specifically binds to the 65-70 kDa common γ subunit (γc) that is shared by the IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21 receptor complexes. The γc receptor is a type 1 transmembrane glycoprotein that is constitutively expressed by most peripheral T and B lymphocytes, NK cells, monocytes and granulocytes. The cytoplasmic domain of the γc chain plays an important role in cytokine-mediated signal transduction. By immunofluorescent staining and flow cytometric analyses, the AG184 antibody has been shown to specifically recognize human γc expressed by cell lines including human γc gene-transfected cell lines which are known to express the human γc chain. The AG184 antibody can bind the γc chain in the receptors complexed with IL-2, IL-4, or IL-7, indicating that the antibody recognizes an epitope which is distinct from the cytokine binding site of the γc chain.

561699 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

561699 Rev.2

Citations & References

Development References (4)

Ishii N, Kondo M, Takeshita T, and Sugamura K. mAb specific for the γ chain of the IL-2 receptor. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1867-1868.
Ishii N, Takeshita T, Kimura Y, et al. Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood. Int Immunol. 1994; 6(8):1273-1277. (Biology). View Reference
Matsuoka M, Takeshita T, Ishii N, Nakamura M, Ohkubo T, Sugamura K. Kinetic study of interleukin-2 binding on the reconstituted interleukin-2 receptor complexes including the human gamma chain. Eur J Immunol. 1993; 23(10):2472-2476. (Biology). View Reference
Nishio J, Kohsaka H, Shimamura T, Hamuro J, Miyasaka N. Abundant expression of common cytokine receptor gamma chain (CD132) in rheumatoid joints.. J Rheumatol. 2001; 28(2):240-4. (Clone-specific). View Reference

View All (4)

561699 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.