The 344823 monoclonal antibody specifically recognizes CD366, which is also known as T cell immunoglobulin mucin 3 (TIM-3), or T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3/TIMD3). CD366 (TIM-3) is a ~60 kDa type I transmembrane glycoprotein that is encoded by HAVCR2 (Hepatitis A virus cellular receptor 2) and belongs to the human TIM family (along with TIM-1 and TIM-4) within the Ig superfamily. CD366 (TIM-3) has one Ig-like V-type domain and a mucin stalk region which is rich in serine and threonine. It is expressed on type-1 (Th1-like) CD4+ T cells and CD8+ T cells, type-17 (Th17-like) CD4+ T cells, regulatory T cells (Treg), NKT, and NK cells. It is also expressed on dendritic cells, mast cells, monocytes, macrophages, and microglia but not on B cells or type-2 (Th2-like) CD4+ T cells. CD366 (TIM-3) can regulate macrophage activation and suppress type-1 and type-17 T cell functions. Crosslinking of cell surface CD366 (TIM-3) by binding to Galectin-9 and/or phosphatidylserine can either positively or negatively regulate leucocyte functions, such as cytokine production or the phagocytosis of apoptotic cells. Through its suppressive activity, CD366 (TIM-3) helps maintain peripheral immune tolerance and homeostasis, but can also inhibit anti-tumor immunity.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
