Alexa Fluor® 647 Mouse Anti-Human GLUT1
Clone 202915 (RUO)
- Brand BD Pharmingen™
- Alternative Name SLC2A1; GLUT-1; Glut; GTR1; PED; HTLVR
- Concentration 0.2 mg/ml
- Isotype Mouse IgG2b, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human GLUT1-transfected cells
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The monoclonal antibody 202915 specifically recognizes Glucose transporter 1 (GLUT1) that is encoded by SLC2A1 (Solute carrier family 2, facilitated glucose transporter member 1). GLUT1 serves as a transporter for glucose and other aldose sugars and oxidized ascorbic acid (vitamin C) into cells. GLUT1 is also known as the Receptor for HTLV-1 and HTLV-2 (HTLVR). Structural studies suggest that GLUT1 is a twelve-pass transmembrane glycoprotein (12TM) with cytoplasmic N- and C-termini. GLUT1 plays an important role in the glycolytic pathway by transporting glucose across the plasma membranes of cells. GLUT1 is differentially expressed on a wide variety of normal cells including erythrocytes, blood-brain barrier endothelial cells, astrocytes, adipocytes, cardiomyocytes, lymphocytes and other leucocytes, as well as by tumor cells and cell lines.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.