Alexa Fluor® 647 Mouse Anti-Human CLM-8 (CD300a)
Clone MEM-260 (RUO)
- Brand BD Pharmingen™
- Alternative Name CD300A; CLM-8; CLM8; CMRF-35-H9; CMRF35H; IGSF12; IRp60; IRC1: IRC2
- Concentration 0.2 mg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen HPB Human Acute Lymphoid Leukemia Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The MEM-260 monoclonal antibody specifically recognizes CD300a which is also known as CMRF35-like molecule 8 (CLM8), Inhibitory receptor protein 60 (IRp60), or Immunoglobulin superfamily member 12 (IGSF12). CD300a is a ~60 kDa type I transmembrane glycoprotein that belongs to the CMRF family within the Ig gene superfamily. CD300a is expressed on natural killer (NK) cells, T and B cell subsets, monocytes, macrophages, dendritic cells, granulocytes and mast cells. CD300a has a single Ig V-like extracellular domain which is homologous to the Ig-binding domains of the of the Fc receptor for polymeric IgA and IgM. CD300a is a non-MHC-specific inhibitory receptor that contains three immunoreceptor tyrosine-based inhibitory motifs in its intracellular region. CD300a can reportedly inhibit NK cell cytolytic activity and granulocyte and mast cell functions.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.