Rainbow Calibration Particles (6 peaks), 3.0 - 3.4 µm
- Brand Sphero™
- Storage Buffer Aqueous solution containing 0.01%NP40 and ≤ 0.02% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
This product contains a mixture of 3.0 - 3.4 µm Rainbow Calibration Particles that are dyed to six different fluorescent intensities. Rainbow Calibration Particles (approx. 1 x 10^7 particles/mL) are designed for routine calibration of flow cytometers and contain a mixture of fluorophores that are excited at any wavelength from 365 - 650 nm. Rainbow Calibration Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis. Before use, mix or resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 mL of sheath fluid will provide an adequate number of particles for flow cytometric analysis.
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.