Alexa Fluor® 700 Mouse anti-Human CD197 (CCR7)
Clone 150503 (RUO)
- Brand BD Pharmingen™
- Alternative Name CC chemokine receptor 7; BLR2; CMKBR7; EBI1; EVI1; MIP-3 beta receptor
- Vol. Per Test 5 µl
- Isotype Mouse IgG2a
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human CCR7 Transfected Cell Line
- Workshop No. VIII 80133
- Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The monoclonal antibody 150503 specifically binds to the human CC chemokine receptor, CCR7, also known as CD197. CCR7 (previously known as BLR2, EBI1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3β/Exodus 3/ELC/ CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL21. CCR7 mRNA is expressed mainly in lymphoid tissues including the spleen, lymph nodes and tonsil. CD197/CCR7 is expressed on peripheral T and B lymphocytes by bone marrow and cord blood CD34-positive cells and by mature dendritic cells. In response to its cognate chemokines, CD197/CCR7 mediates homing of leucocytes to secondary lymphoid tissues. Differential CCR7 expression can be used to distinguish naive, central memory, and effector memory T cell subsets. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17 (region 17q12).
Caution: Under some complex multi-color conditions, this clone may non-specifically interact with antibodies conjugated with APC-H7 or APC-Cy7, contributing to staining artifacts. BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794), designed to minimize on non-specific fluorescent dye interactions, does not resolve this interaction with either APC-H7 or APC-Cy7. For optimal multicolor staining results, alternatives to APC-H7 and APC-Cy7 should be evaluated.
Alexa Fluor® 700 is a far-red dye that can be excited with a 633–640-nm laser. This enables multicolor analysis in conjunction with APC or Alexa Fluor® 647, and APC-H7 or APC-Cy7 reagents.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.