BV421 Mouse Anti-Rat CD3

BD Horizon™ BV421 Mouse Anti-Rat CD3

Name: BV421 Mouse Anti-Rat CD3
Brand: BD Horizon™
SKU: 563948

Clone 1F4

(RUO)

Two-color flow cytometric analysis of CD3 expression on rat splenocytes. Rat splenic leucocytes were stained with PE Mouse Anti-Rat CD5 antibody (Cat. No. 554851) and either BD Horizon™ BV421 Mouse IgM, κ Isotype Control (Cat. No. 562704; Left Panel) or BD Horizon™ BV421 Mouse Anti-Rat CD3 antibody (Cat. No. 563948; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 (or Ig Isotype control staining) versus CD5 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes.

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Product Details

Brand: BD Horizon™

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgM, κ

Immunogen: F344 rat splenic T cells stimulated with PMA plus A23187

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2738503

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Brilliant Violet™ 421 is a trademark of Sirigen.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

BV421 Mouse IgM, κ Isotype Control RUO

Size 50 µg Cat No. 562704

PE Mouse Anti-Rat CD5 RUO

Size 0.2 mg Cat No. 554851

563948 Rev. 1

Antibody Details

1F4

The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

563948 Rev. 1

Format Details

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV421

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 423 nm

563948 Rev.1

Citations & References

Development References (3)

Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Biology). View Reference
Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Immunogen: Flow cytometry, Functional assay, Immunohistochemistry, Stimulation). View Reference

563948 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.