Human Intracellular Cytokine Staining Starter Kit
- Brand BD Pharmingen™
- Reactivity Human (QC Testing)
Intracellular Staining (Routinely Tested)
- Regulatory Status RUO
Regulatory Status Legend
The BD Pharmingen™ Intracellular Cytokine Staining Starter Kit serves as an introduction to the intracellular staining technique. This kit includes all of the reagents and protocols necessary for the researcher to activate human cells in culture and stain these activated cells for intracellular accumulations of IL-2, IFN−γ and TNF. These cytokines are representative of the cytokine response profile elicited by short-term activation of T-cell populations. Individually, IL-2 is a powerful cell growth and differentation factor while IFN−γ and TNF both mediate pro-inflammatory cellular responses. Each serves a key role in short- and long-term immune responses to pathogens and are critical mediators of immune function. (Longer or different activation methods may be required to elicit production of other cytokines and chemokines.)
|BD Pharmingen™ Stain Buffer (FBS)||125 mL||4°C|
|BD Cytofix/Cytoperm™ Buffer||25 mL||4°C|
|BD Perm/Wash™ Buffer||25 mL||4°C|
|PE-conjugated Anti-Human IL-2||25 Tests||4°C|
|PE-conjugated Anti-Human IFN−γ||25 Tests||4°C|
|PE-conjugated Anti-Human TNF||25 Tests||4°C|
|PE-conjugated Isotype Control Cocktail||25 Tests||4°C|
|Purified Blocking Antibody Cocktail||15 Tests||4°C|
|Leukocyte Activation Cocktail||2 vials||-80°C|
|HiCK-1 Cytokine Positive Control Cells||1 vials||-80°C|
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