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Biotin Mouse Anti-Human CD55 IA10 RUO

Biotin Mouse Anti-Human CD55 

Clone  IA10   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name DAF; CR; CROM; TC; Complement decay-accelerating factor
  • Vol. Per Test 20 µl
  • Isotype Mouse IgG2a, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Purified Human Decay Accelerating Factor
  • Workshop No. V BP352, S031
  • Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The IA10 monoclonal antibody specifically binds to CD55, which is also known as complement decay-accelerating factor (DAF). CD55 is a glycophosphatidylinositol (GPI)-anchored, single chain membrane glycoprotein of approximately 70 kDa that belongs to the regulators of complement activation (RCA) gene family which includes CD21, CD35, and CD46. CD55 is widely expressed on hematopoietic cells including platelets and erythrocytes, as well as on many non-hematopoietic cells, such as, endothelial cells and epithelial cells. CD55 is involved in protecting cells from damage by autologous activated complement complexes. CD55 prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertases, C4b2a3b and C3bBb3b.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Format
  • Format Biotin

Other Formats →

Suggested Companion Products

Biotin Mouse IgG2a, κ Isotype Control RUO
100 Tests Cat No: 555572

PE Streptavidin RUO
0.5 mg Cat No: 554061

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Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.


  1. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. View reference

  2. Kinoshita T, Medof ME, Silber R, Nussenzweig V. Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria. J Exp Med. 1985; 162(1):75-92. View reference

  3. Loveland BE, Szokolai K, Johnstone RW, McKenzie IF. Coordinate functions of multiple complement regulating molecules, CD46, CD55, and CD59. Transplant Proc. 1994; 26(3):1070-1071. View reference

  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; .

555692 Rev. 5
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