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Purified Mouse Anti-Human CD14 M5E2 RUO

Purified Mouse Anti-Human CD14 

Clone  M5E2   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein
  • Concentration 0.5 mg/ml
  • Isotype Mouse IgG2a, κ
  • Reactivity Human, Rhesus, Cynomolgus (QC Testing)  Dog (Tested in Development) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Human CD14 Protein
  • Workshop No. II M34; III M329
  • Entrez Gene ID 929 
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The M5E2 monoclonal antibody specifically binds to CD14, a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

Purified Mouse IgG2a, κ Isotype Control RUO
0.1 mg Cat No: 556651

FITC Goat Anti-Mouse IgG/IgM Polyclonal RUO
0.5 mg Cat No: 555988

Lysing Buffer RUO
100 mL Cat No: 555899

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


  1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989; :1-1182.

  2. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987; :1-1050.

  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; .

  4. Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990; 249(4975):1431-1433. View reference

557152 Rev. 2
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