BD™ Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit
- Brand BD™ Cytometric Bead Array (CBA)
- Contents Assay diluent, capture bead diluent, detection reagent diluents, wash buffer, enhanced sensitivity detection reagent, instrument setup reagents
Flow cytometry (Routinely Tested)
- Regulatory Status RUO
Regulatory Status Legend
The BD™ CBA Mouse Enhanced Sensitivity Flex Set System employs particles with discrete fluorescence intensities to detect soluble analytes at very low concentrations. The working assay range for most analytes in this system is 274 to 200,000 fg/mL. The BD CBA Mouse Enhanced Sensitivity Master Buffer Kit (Catalog Nos. 562246 and 562248) contains all of the supporting reagents necessary to perform an assay using a BD CBA Mouse Enhanced Sensitivity Flex Set. The buffers, Enhanced Sensitivity Detection Reagent (Part B), and instrument setup reagents provided in this kit have been optimized for analysis of analytes in tissue culture supernatants and serum samples. The BD CBA Mouse Enhanced Sensitivity Master Buffer Kit provides sufficient reagents for the quantitative analysis of 100 samples and 10 instrument setup procedures (Catalog No. 562246) or 500 samples and 10 instrument setup procedures (Catalog No. 562248).
Reagent Quantity (562246) Quantity (562248) Assay Diluent 1 bottle, 30 mL 1 bottle, 150 mL Capture Bead Diluent 1 bottle, 5 mL 1 bottle, 30 mL Detection Reagent Diluent A 1 bottle, 3 mL 1 bottle, 15 mL Detection Reagent Diluent B 1 bottle, 20 mL 2 bottles, 35 mL each Wash Buffer 1 bottle, 250 mL 2 bottles, 650 mL each Enhanced Sensitivity Detection Reagent (Part B) 2 vials, lyophilized, 50 tests each 10 vials, lyophilized 50 tests each Instrument Setup Bead A1 1 vial, 0.25 mL 1 vial, 0.25 mL Instrument Setup Bead A9 1 vial, 0.25 mL 1 vial, 0.25 mL Instrument Setup Bead F1 1 vial, 1.0 mL 1 vial, 1.0 mL Instrument Setup Bead F9 1 vial, 0.25 mL 1 vial, 0.25 mL PE Instrument Setup Bead F1 1 vial, 0.25 mL 1 vial, 0.25 mL PE Positive Control Detector 1 vial, 0.5 mL 1 vial, 0.5 mL
Preparation and Storage
Store all kit components at 2 to 8°C. Do not freeze.
This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
The BD CBA Human IL-1β Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-1β Flex Set will be 2,500 pg/ml. An example standard curve is shown in Figure 1.
The BD CBA Human IL-1β Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset.
Limit of Detection: The theoretical limit of detection is 2.3 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.