Human IL-8 ELISA Kit II
- Brand BD OptEIA™
- Reactivity Human (QC Testing)
ELISA (Routinely Tested)
- Regulatory Status RUO
Regulatory Status Legend
Interleukin-8 (IL-8) is a member of a family of structurally-related low molecular weight proinflammatory factors known as chemokines. Alternative designations for IL-8 include neutrophil attractant protein-1 (NAP-1), neutrophil activating factor (NAF), monocyte-derived neutrophil chemotactic factor (MDNCF), leukocyte adhesion inhibitor (LAI), monocyte derived neutrophil-activating peptide (MONAP) and granulocyte chemotactic protein (GCP). Although it was initially described as a chemotactic factor produced by activated monocytes, IL-8 has since been found to be produced by a variety of different cell types including monocytes, T lymphocytes, endothelial cells, epithelial cells, smooth muscle cells, and fibroblasts. Autocrine production by neutrophils also occurs under certain conditions.
The BD OptEIA™ ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance “Recovery” and “Linearity” sections.
The BD OptEIA Human IL-8 ELISA Kit II is for the quantitative determination of human IL-8 in serum, plasma, and cell culture supernatant.
|Antibody Coated Wells||2 plates (12 strips x 8 wells)|
|Detection Antibody||30 mL|
|Standards||4 vials, lyophilized|
|Enzyme Concentrate (250x)||150 µL|
|Standard/Sample Diluent||30 mL|
|ELISA Diluent||12 mL|
|Wash Concentrate (20x)||100 mL|
|TMB One-Step Substrate Reagent||30 mL|
|Stop Solution||13 mL|
|Plate Sealers||4 each|
Preparation and Storage
1. Store kit at 2 – 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 – 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 – 8°C).
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