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FlowJo™ v11 Software
Overview
The leading analysis platform for single-cell flow and mass cytometry analysis
FlowJo™ Software is the premier platform for single-cell flow cytometry analysis and provides a rich analysis environment that is easy to use, versatile and extensible.
- Compatible with standard FCS data files on both Mac and PCs
- Intuitive drag-and-drop analysis
- Advanced data analysis algorithms
- Convenient visualization tools to minimize time to insight
- Supported by industry-leading education and unparalleled support
Experience the Future of Data Analysis with FlowJo™ v11 Software
Discover the future of flow cytometry data analysis with FlowJo™ v11 Software, brought to you by the trusted and experienced scientists you know.
FlowJo™ v11 Software brings a wide range of innovative features providing users with an immersive experience for advanced data analysis. FlowJo™ v11 Software allows for faster performance while maintaining its rich feature set for a variety of users.
FlowJo™ v11 Software includes:
- Immersive user experience for intuitive navigation: Featuring an all-in-one interface that requires no additional plugin setup, allowing you to perform your analysis quickly and efficiently
- Faster performance for reducing time to discovery: Enhanced algorithm speed for quicker analysis of larger data sets and built in charts, tables and differential statistics
- Built-in features for added workflow efficiency: Framework designed to enable comparison, built-in charts, and many of your favorite algorithms integrated into the design, offering analysis tools for QC, clustering, dimensionality reduction, image analysis, and more
Data Preprocessing
- Data preprocessing with built-in QC
- Focus on parameters of interest with parameter sets
- Resolve slight mismatches in names to align equivalent fluorochromes with parameter flexibility
- Optimized concatenation workflow with virtual concatenation
- Focus on parameters of interest with parameter sets
Data Preprocessing with Built-in QC
Compensation and Unmixing
- Fully featured Unmixing / Compensation platform
- Spectral unmixing and traditional compensation
- AutoSpill and Gated-Based approaches
- Apply unmixing parameters to specific samples in the analysis tree
- Spectral unmixing and traditional compensation
- Streamlined workflow
- Make precise adjustments to spectral matrices with our integrated adjustment matrix
- Assess matrices more easily with a refined preview pane, and a suite of visualization tools.
- Make precise adjustments to spectral matrices with our integrated adjustment matrix
Data Compensation and Unmixing
Data Comparison and Visualization
- Compare data with a click of a button:
- Compare across different samples with the graph gallery
- Generate tables and charts
- View differential statistics
- Compare across different samples with the graph gallery
- Algorithmic analysis
- tSNE dimensionality reduction
- x-shift clustering analysis
- tSNE dimensionality reduction
- Visualize imaging data easily
- Integrated image wall that mirrors what you see in BD FACSChorus™ Software
Data comparison and visualization
Understand The New Functions of FlowJo™ v11 Software
| Features | FlowJo™ v10 Software | FlowJo™ v11 Software |
|---|---|---|
| Mac and Windows compatible | √ | √ |
| Graphical user interface with interactive samples | √ | √ |
| Grouping functionality | √ | √ |
| Hierarchical grouping | ||
| Manual gating | √ | √ |
| Basic statistics | √ | √ |
| Batch processing by sample | √ | √ |
| Batch processing by population | √ | |
| Summary plots (bar, pie) | √ | |
| Templates | √ | |
| Page-based reports | √ | |
| Parameter sets | √ | |
| Export report as images (jpeg, tiff, svg) | √ | |
| Full suite of compensation and unmixing tools | √ | √ |
| Unmixing matrix correction | √ | |
| QC tools | As Plugin | √ |
| Models (Kinetics, Proliferation, Cell cycle) | √ | |
| Comparative statistics | √ | |
| Parameter math | √ | |
| File concatenation | √ | |
| Virtual concatenation | √ | |
| X-Shift | As Plugin | √ |
| tSNE | √ | √ |
| Dimensionality reduction with downsampling and upsampling | √ |
Licensing and Access:
Available through our user-based subscription licensing system, BD Access Portal (formerly FlowJo Portal), provides automatic access to new version releases, account management features, flexible cloud and user-based licenses (no physical dongles) and administrative tools.
- Automatic Updates: Always have access to the latest version of the software with new features and improvements.
- Flexibility: User-based licenses eliminate the need for physical dongles, allowing you to log in with an email account from anywhere.
- Account Management: Enhanced tools for group administrators to manage users, initiate trial periods, and handle subscriptions.
"FlowJo™ v11 Software is game-changing. The auto-QC feature allows reproducible downstream data analysis, while the all-in-one viewing option makes gating application across samples and groups quick and easy. More importantly, the graphing feature allows streamlined analysis to visualization, reducing time from acquisition to presentation."
Dr. Logan S. Dean
Doctoral Researcher
Colorado State University
FlowJo™ v10 Software
Overview
For customized workflows unique to the rich feature set of FlowJo™ v10 Software.
FlowJo™ Software is your partner and committed to take your research to new heights.
FlowJo™ Software enables single-cell flow cytometry analysis that helps unlock your data insights with accessible features for immunophenotyping, cell cycle, proliferation, kinetics studies, quantitative population comparison, plate screening assays and more.
With an intuitive interface, specialized analysis platforms and open-ended plugin architecture, FlowJo™ v10 Software provides a rich analysis environment that is easy to use, versatile and extensible.
Features
The latest release of FlowJo™ v10.10 Software
- Data visualization: Create spectral plots for selected detectors and populations and compare populations using spectral plots with Spectral Population Viewer
- NxN plot parameter mouseover: Display parameter combinations on NxN plots for easier viewing
- Advanced Scaling Preferences: Define data-driven, per-cytometer scaling defaults to more easily set transform properties
- Added functionality for MEM, ClusterExplorer, the compensation editor and more!
Open-ended plugin architecture helps your research stay ahead of the curve. FlowJo™ Software offers easy plugin installation that brings ongoing informatics innovation to your data visualizations.
Our developers work with the brightest minds in the industry to cultivate these cutting-edge resources. Current offerings include but are not limited to:
- Phenograph
- ClusterExplorer
- UMAP
- CytoNorm
- FlowSOM
Download and see the complete library of plugins available on the FlowJo Exchange!
With BD Access Portal subscription packages, you can access the version(s) that suits your needs
Whether you require specific features unique to FlowJo™ v10 Software or are ready to explore the latest technologies with FlowJo™ v11 Software.
Learn more about which version of FlowJo has the features that meets your needs
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD, the BD Logo, BD FACSChorus and FlowJo are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners.