Description
Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells. Recombinant human MCP-1 (Cat. No. 554620) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant human MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng/µg of human MCP-1, as measured in a chromogenic LAL assay.
Preparation And Storage
Recommended Assay Procedures
Upon initial thawing, recombinant human MCP-1 (Cat. No. 554620) should be aliquoted into polypropylene microtubes and frozen at -80˚C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine albumin, aliquoted, and stored at -80°C. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/ mL are recommended. For in vitro biological assays, carrier-protein concentrations ≥ 0.5 mg/mL are suggested. Carrier proteins should be pre-screened for possible effects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Human MCP-1 is useful as a quantitative standard for measuring human MCP-1 protein levels in a MCP-1 specific sandwich ELISA with the purified 10F7 antibody (Cat. No. 555055) as a capture antibody and the biotinylated clone 5D3-F7 (Cat. No. 554664) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human MCP-1 standard from ~ 2000 to 4 pg/mL should be included with each ELISA plate. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems, not for assay of serum or plasma samples. For measuring MCP-1 in serum or plasma, the BD OptEIA™ Human MCP-1 Set (Cat. No. 555179) is specially formulated and recommended.
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. Activity encompassing an EC50= 20 - 350 ng/mL has previously been reported using THP-1 indicator cells for proliferation.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Development References (5)
-
Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Biology). View Reference
-
Rollins BJ, Stier P, Ernst T, Wong GG. The human homolog of the JE gene encodes a monocyte secretory protein. Mol Cell Biol. 1989; 9(11):4687-4695. (Biology). View Reference
-
Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). View Reference
-
Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p.
-
Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 1989; 244(2):487-493. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
