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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
ELISA Capture: The purified 10F7 antibody is useful as a capture antibody for a sandwich ELISA for measuring human MCP-1 protein levels. The purified 10F7 antibody can be paired with the biotinylated anti-human MCP-1 monoclonal antibody 5D3-F7 (Cat. No. 554664) as the detection antibody with recombinant human MCP-1 (Cat. No. 554620) as the standard. Purified 10F7 antibody should be titrated 2-6 µg/ml to determine the optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human MCP-1 ranging from ~5000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the chapter on ELISA in the Immune Function Handbook, which is posted on our website at www.bdbiosciences.com.
Note 1: This ELISA pair shows no cross-reactivity with any of the following chemokines tested (e.g., Human eotaxin, MCP-2, MCP-3, MCP-4, mouse MCP-1 and rat MCP-1).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum samples. BD OptEIA™ ELISA Set Cat. No. 555179 and Kit 559017 are specially-formulated for serum cytokine measurement.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
The 10F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF). The immunogen used to generate the 10F7 hybridoma was E. coli-expressed recombinant human MCP-1.
This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (2)
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Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Biology). View Reference
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Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 1989; 244(2):487-493. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.