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BD Horizon™ Human T Cell Backbone Panel Kit

Expand your T cell research with confidence

The BD Horizon™ Human T Cell Backbone Panel consists of an optimized configuration of core markers specifically designed to expand your research with recommended fluorochrome drop-ins and enable flexibility in panel design without compromising resolution.

Fluorochromes were strategically selected so that up to five recommended drop-ins can be added with minimal impact to the backbone resolution and minimal panel design effort, allowing you to focus on marker selection.

Learn more from the BD Horizon™  Human T cell backbone panel brochure.

 
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Watch the webinar describing the use of the BD Horizon™ T Cell Backbone Panel for expanding your T cell research.

The BD Horizon™ Human T Cell Backbone Panel contains a set of markers that can be combined with up to five additional fluorochromes without impacting resolution
 

Marker Clone Fluorochrome
CD3 UCHT1 BV510
CD4 SK3 BV786
CD8 RPA-T8 R718
CD45RA HI100 PE-Cy7
CD197 (CCR7) 2-L1-A BV711

BD Horizon™ Brilliant Stain Buffer is included to provide optimal reagent performance.

Examples of recommended drop-in fluorochromes
 

Laser Drop-in 
Violet 405 nm BV421/V450/Pacific Blue™
Blue 488 nm FITC/BB515/Alexa Fluor™ 488/PE
Yellow-Green
561 nm
PE/RY586
Red 640 nm APC/Alexa Fluor™ 647
UV 355 nm BUV395

Drop-in reagents not included in backbone kit.

AF: Alexa Fluor™ Reagent

BB: BD Horizon Brilliant™ Blue Reagent, BUV: BD Horizon Brilliant™ Ultraviolet Reagent

BV: BD Horizon Brilliant™ Violet Reagent, R: BD Horizon™ Red Reagent

RY: BD Horizon RealYellow™ Reagent

The BD Horizon RealYellow™ (RY) 586 Fluorochrome can be used as an alternative to PE when excited off the yellow-green laser. Simultaneous use of PE off the blue laser and RY586 off the yellow-green laser is not recommended. 

The BD Horizon™ Human T Cell Backbone Panel offers increased flexibility and fewer design challenges

Clear resolution of five core markers defining major naive and memory subsets of CD4+ and CD8+ T cells
Verified flexibility enabling deeper investigation of different facets of T cell biology (activation, differentiation, polarization, Treg immunophenotype)
Compatible with buffers and protocols used for intracellular detection of cytokines, transcription factors or phosphorylated proteins

High Performance

  • Strategically designed to be complemented with up to five drop-in markers with no resolution impact to the backbone or the selected drop-ins
  • Verified protocols to ensure optimal performance
  • Consistent performance across instruments with different optical configurations
 
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BD flow cytometers are Class 1 Laser Products.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Alexa Fluor and Pacific Blue are trademarks of Thermo Fisher Scientific. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.