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Phosflow_Protocol_for_Human_PBMCs.pdf

Methods and kinetics of activation vary for each phosphorylated cell signaling molecule. Select appropriate stimuli and stimulation times before beginning the protocol.

BD-Protocol-Freezing-PBMCs.pdf

Cryopreserved PBMCs are a common specimen source for future phenotypic and functional analyses. It is important to have an optimized protocol for freezing and thawing PBMCs to retain viability and function for future analyses by flow cytometry.

560746.pdf

Perm Buffer IV 10× RUO - TDS 560746

569343.pdf

Alexa Fluor™ 647 Mouse Anti-Human CPT1A 8F6AE9 RUO - TDS 569343

Phosflow-96-Well-Deep-Well-Plate-Protocol.pdf

Reagents required, suggested equipment, and procedural notes.

Phosflow_Protocols_for_TCR_Stimulation_Human.pdf

Phosphorylation varies among cell signaling molecules and cell types. Many of the events induced by T-cell receptor stimulation are max within 1 to 15 min, with phosphorylation decreasing rapidly afterwards.

Human_Reg_Tcell_Isolation_Measurement.pdf

In this application note we demonstrate the performance and utility of theBD Pharmingen™ human regulatory T cell sorting kit to sort CD45RA+ and CD45RA– Treg populations using a BD FACSAria™ II cell sorter.

Aria_III_Adv_of_561_nm_app_note.pdf

Proof of principle data presented in this application note demonstrate the advantages of using the 561-nm laser to excite and detect PE-conjugated antibodies and mCherry-ex

BD-Rhapsody-Single-Cell-Analysis-System.pdf

BD Rhapsody™ Scanner Software Installation Guide

BD-Rhapsody-Single-Cell-Analysis-System-Instrument.pdf

BD Rhapsody™ Scanner Software Installation Guide

BD-Rhapsody-Single-Cell-Suspension-User-Guide.pdf

This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.