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Purified Mouse anti-p130Cas (pY249)
Purified Mouse anti-p130Cas (pY249)

Western blot analysis of p130Cas (pY249) in human Burkitt's lymphoma.  Lysates from control (left panel) and hydrogen peroxide-activated (right panel) Ramos cells were probed with purified mouse anti-p130Cas (pY249) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.0312 µg/ml (lanes 3 and 6).  p130Cas (pY249) is identified as a band of 130 kDa in the treated cells.

Western blot analysis of p130Cas (pY249) in human Burkitt's lymphoma.  Lysates from control (left panel) and hydrogen peroxide-activated (right panel) Ramos cells were probed with purified mouse anti-p130Cas (pY249) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.0312 µg/ml (lanes 3 and 6).  p130Cas (pY249) is identified as a band of 130 kDa in the treated cells.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2b, κ
Phosphorylated Human p130Cas
Western blot (Routinely Tested)
130 kDa
0.5 mg/ml
AB_647286
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558401 Rev. 2
Antibody Details
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J169-757.12.2

p47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses.  The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains.  v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate).  The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk.  Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk.  This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus.  Since p130Cas also associates with v-Src, it may be a v-Src substrate.  Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule.

The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas.  The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.

558401 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558401 Rev.2
Citations & References
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Development References (2)

  1. Goldberg GS, Alexander DB, Pellicena P, Zhang Z-Y, Tsuda H, Miller WT. Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells. J Biol Chem. 2003; 278(47):46533-46540. (Biology).
  2. Hinsby AM, Olsen JV, Bennett KL, Mann M. Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry. Mol Cell Proteomics. 2003; 2(1):29-36. (Biology).
558401 Rev. 2

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