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Purified Mouse Anti-Bax
Purified Mouse Anti-Bax
Western blot analysis of Bax. Lysates from Daudi human B cells (lane 1) and mouse thymocytes (lane 2) were probed with anti-Bax (clone 6A7, Cat. No. 556467) Clone 6A7 identifies Bax as an ~21 kDa band.
Western blot analysis of Bax. Lysates from Daudi human B cells (lane 1) and mouse thymocytes (lane 2) were probed with anti-Bax (clone 6A7, Cat. No. 556467) Clone 6A7 identifies Bax as an ~21 kDa band.
Product Details
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BD Pharmingen™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1, κ
N-terminal peptide sequence
Western blot (Routinely Tested), Immunoprecipitation (Reported)
21-22 kDa
0.5 mg/ml
AB_396430
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1-2 µg/ml)  and immunoprecipitation. Daudi B lymphoma cells (ATCC CCL-213) and rat thymocytes are suggested as positive controls for western blot analysis. Due to low level of Bax expression in Hela cells, we do not recommend Hela as a positive control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556467 Rev. 8
Antibody Details
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6A7

The Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2).  Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax may form homodimers or heterodimers with either Bcl-2 and Bcl-XL (long). Bax homodimers is thought to promote cell death, whereas Bax heterodimerization with either Bcl-2 and Bcl-XL appears to block cell death. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. Bax expression has been identified in a variety of tissues, including lung, stomach, kidney, thymus, lymph nodes, bone marrow, spleen, heart, exocrine pancreas, and brain.  Liver, kidney, and the exocrine pancreas have been found to contain little or no Bcl-2. The Bax gene potentially encodes three different proteins Bax-α (21 kDa), Bax-β (24 kDa) and Bax-γ (5 kDa). Bax-α is the most common transcript.

The 6A7 antibody recognizes human, mouse and rat Bax. The 6A7 antibody reacts with an epitope between  amino acids 12-24 of Bax, and studies suggest that this epitope may be in the  vicinity of the dimerization domain of Bax.  It has been proposed that the 6A7 antibody may directly compete with either Bcl-2 or Bcl-XL for binding to Bax.  In  western blots, the 6A7 antibody typically detects Bax as a single band of 21-22 kDa, thought to be Bax-α.

556467 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556467 Rev.8
Citations & References
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Development References (6)

  1. Hsu YT, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A. 1997; 94(8):3668-3672. (Clone-specific: Western blot). View Reference
  2. Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997; 272(21):13829-13834. (Clone-specific: Western blot). View Reference
  3. Krajewski S, Krajewska M, Shabaik A, Miyashita T, Wang HG, Reed JC. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2. Am J Pathol. 1994; 145(6):1323-1336. (Biology). View Reference
  4. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Biology). View Reference
  5. Reed JC. Bcl-2 and the regulation of programmed cell death. J Cell Biol. 1994; 124(1-2):1-6. (Clone-specific). View Reference
  6. Yin XM, Oltvai ZN, Korsmeyer SJ. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature. 1994; 369(6478):321-323. (Biology). View Reference
View All (6) View Less
556467 Rev. 8

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.