The 25-9-17 monoclonal antibody specifically recognizes the β chain of the I-A[b] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[q] haplotypes. Reactivity with other haplotypes (eg, a, f, g7, k, r, s) has not been observed. The strain distribution of the antigen recognized by this reagent is similar or identical to that of anti-I-A[d] (Aβ[d]) mAb 34-5-3.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.