The ACT5 monoclonal antibody specifically binds to the mouse Toll-like receptor 5 (TLR5), which is also known as CD285. TLR5 is a type I transmembrane glycoprotein and a member of the Toll-like receptor (TLR) family. It is expressed on the surface of monocytes, dendritic cells, neutrophils, and mucosal epithelial cells. TLR5 is a pattern recognition receptor that binds to bacterial flagellin. TLR5 signaling leads to the activation of cells that produce proinflammatory cytokines and chemokines, such as IL-6 and MCP-1. TLR5 thus plays a major role in initiating innate and adaptive immune responses.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.