The M-L13 monoclonal antibody specifically binds to a 24 kDa type III transmembrane protein that is expressed on platelets, pre-B cells, monocytes, endothelial and epithelial cells. CD9 belongs to a family of membrane proteins called tetraspanins that transverse the membrane four times. CD9 is weakly expressed on resting mature B cells. M-L13 induces platelet aggregation and activation. This antibody is also suitable for staining acetone-fixed, frozen tissue sections.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system or other oligo-dT-based capture systems. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.