The AC1.2 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or GMP-140. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin may mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.