The HIP1 monoclonal antibody specifically binds to CD42b. CD42b is also known as the Platelet glycoprotein Ib alpha chain that is encoded by the GP1BA gene. CD42b is disulfide bonded to CD42c to form a 170 kDa heterodimer, GPIb. GPIb forms a noncovalent complex with CD42a and CD42d (CD42 complex) that is expressed on platelets and megakaryocytes. The CD42 complex serves as the von Willebrand Factor (vWF) surface receptor involved in the adhesion of platelets to the subendothelium of damaged vascular walls. HIP1 inhibits the ristocetin-dependent binding of vWF to platelets and partially inhibits collagen-induced aggregation.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.