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    Oligo Mouse Anti-Human BDCA-2 (CD303)

    BD™ AbSeq Oligo Mouse Anti-Human BDCA-2 (CD303)

    Clone V24-785 (RUO)

    Product Details
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    BD™ AbSeq
    CLEC4C; DLEC; HECL; BDCA2; CLECSF7; CLECSF11; PRO34150
    170482
    2 µl
    Mouse BALB/c IgG2b, κ
    Human (Tested in Development)
    Single Cell 3' Sequencing (Qualified)
    TAAGGTAGGCAGTAGATAACGGGACGAATGATGAGC
    AHS0204
    Human BDCA-2 Recombinant Protein
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO
    Mouse


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.
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    Recommended Assay Procedures

    Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.

    BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.

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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
    5. Illumina is a trademark of Illumina, Inc.
    6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
    7. Please refer to bd.com/genomics-resources for technical protocols.
    8. For U.S. patents that may apply, see bd.com/patents.
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    940282 Rev. 2
    Antibody Details
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    V24-785

    The V24-785 monoclonal antibody binds specifically to BDCA-2 (Blood Dendritic Cell Antigen 2), also known as CD303. As a member of the C-type lectin (CLEC) superfamily, it is an adhesion receptor that binds to galactose-terminated desialylated glycans on cell surfaces and on serum IgG. In the blood, BDCA-2 is expressed only on plasmacytoid dendritic cells (pDC). TLR-9 mediates the production of large amounts of IFN-α and IFN-β by activated pDC, and crosslinking of BDCA-2 inhibits that response.

    940282 Rev. 2
    Format Details
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    Antibody-Oligo
    The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD® AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD® AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.NOTE: The BD Rhapsody™ Single-Cell Analysis System must be used with the BD Rhapsody™ Express Instrument.
    Antibody-Oligo
    940282 Rev.2
    Citations & References
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    View product citations for antibody "940282" on CiteAb

    Development References (7)

    1. Dzionek A et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.. J Immunol. 2000; 165(11):6037-6046. (Biology). View Reference
    2. Dzionek A, Inagaki Y, Okawa K, et al. Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions.. Hum Immunol. 2002; 63(12):1133-48. (Biology). View Reference
    3. Dzionek A, Sohma Y, Nagafune J, et al. BDCA-2, a novel plasmacytoid dendritic cell-specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon alpha/beta induction.. J Exp Med. 2001; 194(12):1823-34. (Biology). View Reference
    4. Jégouzo SA, Feinberg H, Dungarwalla T, Drickamer K, Weis WI, Taylor ME. A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2.. J Biol Chem. 2015; 290(27):16759-71. (Biology). View Reference
    5. Riboldi E, Daniele R, Parola C, et al. Human C-type lectin domain family 4, member C (CLEC4C/BDCA-2/CD303) is a receptor for asialo-galactosyl-oligosaccharides.. J Biol Chem. 2011; 286(41):35329-33. (Biology). View Reference
    6. Shinde R, Hezaveh K, Halaby MJ, et al. Apoptotic cell-induced AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans.. Nat Immunol. 2018; 19(6):571-582. (Clone-specific: Fluorescence activated cell sorting). View Reference
    7. Ziegler-Heitbrock L, Ancuta P, Crowe S, et al. Nomenclature of monocytes and dendritic cells in blood.. Blood. 2010; 116(16):e74-80. (Biology). View Reference
    View All (7) View Less
    940282 Rev. 2

    Please refer to Support Documents for Quality Certificates

     

    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

     

    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.