Skip to main content Skip to navigation
Purified Mouse anti-Rb (pS780)
Purified Mouse anti-Rb (pS780)

Western blot analysis of Rb (pS780) in human embryonic skin cells.  Lysates from serum-starved (left panel) and fetal bovine serum-stimulated (right panel) WS1 cell line were probed with purified mouse anti-Rb (pS780) monoclonal antibody at concentrations of 4.0 (lanes 1 and 4), 2.0 (lanes 2 and 5), and 1.0 µg/ml (lanes 3 and 6).  Rb (pS780) is identified as a band of 110 kDa in the stimulated cells.

Purified Mouse anti-Rb (pS780)

Rb (pS780) staining on tonsil.  Fresh human tonsil, stimulated with in 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column).  The tissue sections were stained with purified Mouse anti-Rb (pS780) monoclonal antibody with Hematoxylin counterstaining.  Original magnification: 20X.

Western blot analysis of Rb (pS780) in human embryonic skin cells.  Lysates from serum-starved (left panel) and fetal bovine serum-stimulated (right panel) WS1 cell line were probed with purified mouse anti-Rb (pS780) monoclonal antibody at concentrations of 4.0 (lanes 1 and 4), 2.0 (lanes 2 and 5), and 1.0 µg/ml (lanes 3 and 6).  Rb (pS780) is identified as a band of 110 kDa in the stimulated cells.

Rb (pS780) staining on tonsil.  Fresh human tonsil, stimulated with in 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column).  The tissue sections were stained with purified Mouse anti-Rb (pS780) monoclonal antibody with Hematoxylin counterstaining.  Original magnification: 20X.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Human (QC Testing)
Mouse BALB/c IgG1, κ
Phosphorylated Human Rb
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
110 kDa
0.5 mg/ml
AB_647294
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558385 Rev. 2
Antibody Details
Down Arrow Up Arrow
J146-35

The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors.  Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity.  Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle.  During G1 phase, Rb is predominantly in a hypophosphorylated state.  It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs.  Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46.  Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins.  Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation.  For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription.  Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined.  Thus, Rb appears to regulate transcription in its aim to control cell growth.

The J146-35 monoclonal antibody recognizes Rb phosphorylated at serine 780 (pS780), which affects Rb binding to E2F.  The orthologous phosphorylation sites in mouse and rat Rb are serines 773 and 751, respectively.

558385 Rev. 2
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558385 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (2)

  1. Cobrinik D. Pocket proteins and cell cycle control. Oncogene. 2005; 24:2796-2809. (Biology).
  2. Knudsen ES, Wang JY. Dual mechanisms for the inihibition of E2F binding to RB by cyclin-dependent kinase-mediate RB phosphorylation. Mol Cell Biol. 1997; 17(10):5771-5783. (Biology).
558385 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.