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Purified Mouse Anti-Human Cyclin D1
Purified Mouse Anti-Human Cyclin D1

Western blot analysis of Cyclin D1. MCF7 cell lysates (Human breast adenocarcinoma; ATCC HTB-22) was probed with the mouse anti-human Cyclin D1 antibody (clone DCS-6) at 1-2 µg/mL (Lane 1). Cyclin D1 is identified as a band of ~36 kDa.

Western blot analysis of Cyclin D1. MCF7 cell lysates (Human breast adenocarcinoma; ATCC HTB-22) was probed with the mouse anti-human Cyclin D1 antibody (clone DCS-6) at 1-2 µg/mL (Lane 1). Cyclin D1 is identified as a band of ~36 kDa.

Product Details
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BD Pharmingen™
Human (QC Testing), Mouse, Rat (Reported)
Mouse IgG2a
Human Full-length Cyclin D1 Recombinant Protein
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunoprecipitation (Reported), Intracellular staining (flow cytometry) (Not Recommended)
36 kDa
0.5 mg/ml
AB_396432
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556470 Rev. 9
Antibody Details
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DCS-6

Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.

556470 Rev. 9
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556470 Rev.9
Citations & References
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Development References (4)

  1. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry. 1996; 25(1):1-13. (Biology).
  2. Lukas J, Pagano M, Staskova Z, Draetta G, Bartek J. Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines. Oncogene. 1994; 9(3):707-718. (Immunogen: Immunohistochemistry, Immunoprecipitation, Inhibition, Western blot). View Reference
  3. Meyerson M, Harlow E. Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 1994; 14(3):2077-2086. (Biology). View Reference
  4. de Boer CJ, Schuuring E, Dreef E, et al. Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma. Blood. 1995; 86(7):2715-2723. (Biology: Immunohistochemistry). View Reference
View All (4) View Less
556470 Rev. 9

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.