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Western blot analysis of Dystrobrevin on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dystrobrevin.
Immunofluorescent staining of Rabbit Cerebrum.
BD Transduction Laboratories™ Purified Mouse Anti-Dystrobrevin
BD Transduction Laboratories™ Purified Mouse Anti-Dystrobrevin
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Acetylcholine receptor clustering is considered an early event and a marker for synaptogenesis between a motor neuron and a muscle fiber. This process is mediated by the extracellular protein Agrin which binds to α and β Dystroglycan, previously discovered as Dystrobrevin- associated proteins. Dystrobrevin binds to the actin cytoskeleton by its N-terminal domain, thus providing the link between the actin cytoskeleton and receptor clustering. In Torpedo electric organ, Dystrobrevin is a prominent phosphotyrosine-containing protein. Murine Dystrobrevins comprise a family of four related proteins (1-4) with similar function, generated by alternative splicing. Dystrobrevin-1 is 688 amino acids and has an apparent mobility of 87kDa. Dystrobrevin-2 shares almost identical sequence with Dystrobrevin-1 in the first 500 amino acids and has an apparent mobility of 59kDa.
Development References (1)
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Blake DJ, Nawrotzki R, Peters MF, Froehner SC, Davies KE. Isoform diversity of dystrobrevin, the murine 87-kDa postsynaptic protein. J Biol Chem. 1996; 271(13):7802-7810. (Biology). View Reference
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