Skip to main content Skip to navigation
AEC Substrate Kit

AEC Substrate Kit

(RUO)
AEC Substrate Kit

Immunohistochemical staining with AEC substrate. Formalin-fixed paraffin sections of human tonsil were reacted with the anti-human CD45RO antibody and the color developed using Streptavidin-Horse Radish peroxidase and AEC substrate. Counterstain used is contrast green. Magnification 40x.

Immunohistochemical staining with AEC substrate. Formalin-fixed paraffin sections of human tonsil were reacted with the anti-human CD45RO antibody and the color developed using Streptavidin-Horse Radish peroxidase and AEC substrate. Counterstain used is contrast green. Magnification 40x.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Immunohistochemistry (Routinely Tested)
RUO
AB_2868917


Description

3-Amino-9-Ethylcarbazole (AEC) is a substrate for peroxidase and is a widely used chromogen for immunohistochemical staining and immunoblotting. In the presence of peroxidase enzyme, AEC produces a red end product that is soluble in alcohol and therefore must be used with an aqueous counterstain and mounting media.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Immunohistochemistry: Just before staining add one drop of AEC chromogen for every 1 mL of AEC buffer. Apply the AEC substrate reagent to the sections after incubation with horseradish peroxidase and washing is complete. Incubate sections with AEC reagent for 5-20 minutes or until the desired color intensity is obtained. Use the AEC substrate reagent within 20 minutes of mixing.

Warning:  AEC buffer (component 51-7603KC) contains 7.99% methanol (w/w).

Hazard statements

Combustible liquid.

Harmful if swallowed.

May be harmful in contact with skin.

May cause damage to organs.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.

IF ON SKIN: Wash with plenty of water.

Warning:  AEC Chromogen (component 51-7604KC) contains 97.8% N,N-dimethylformamide (w/w).

Hazard statements

Flammable liquid and vapour.

Harmful if inhaled.

Causes serious eye irritation.

May damage fertility or the unborn child.

Precautionary statements

Keep away from heat/sparks/open flames/hot surfaces. - No smoking.

Wear protective clothing / eye protection.

Wear protective gloves.

IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.  Continue rinsing.

Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
551015 Rev. 3
Components
Down Arrow Up Arrow
Description Quantity/Size Part Number
AEC Chromogen (component of AEC substrate Kit) 1.5 mL (1 ea) 51-7604KC
AEC buffer (component of AEC substrate Kit) 50 mL (1 ea) 51-7603KC
551015 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.