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Purified Mouse Anti-Yes
Purified Mouse Anti-Yes

Western blot analysis of Yes on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-Yes antibody.

Purified Mouse Anti-Yes

Immunohistochemical staining on a rabbit brain tissue section with the Mouse Anti-Yes antibody.

Western blot analysis of Yes on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-Yes antibody.

Immunohistochemical staining on a rabbit brain tissue section with the Mouse Anti-Yes antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken, Dog (Tested in Development)
Mouse IgG1
Human Yes aa. 10-193
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development)
62 kDa
250 µg/ml
AB_397759
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610375 Rev. 2
Antibody Details
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1/Yes

Yes is a member of the Src family of tyrosine kinases. The cellular gene was initially identified as a homologue of v-yes, the oncogene of avian sarcoma virus Y73. At least nine different Src family members have been identified. yes, fyn, and Src are widely expressed in a variety of cell types, while the remaining members are primarily expressed in hematopoietic cells. Members of this family have several common features: they have unique N-terminal domains, they attach to cellular membranes through a myristylated N-terminus and they have homologus SH2, SH3, and catalytic domains. The common expression patterns of Yes, fyn, and Src suggest some overlapping functions. All three of these kinases can physically associate with a number of cell-surface molecules which appear to increase their catalytic activity.

610375 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610375 Rev.2
Citations & References
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Development References (5)

  1. Chen YH, Lu Q, Goodenough DA, Jeansonne B. Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells. Mol Biol Cell. 2002; 13(4):1227-1237. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  2. Encinas M, Tansey MG, Tsui-Pierchala BA, Comella JX, Milbrandt J, Johnson EM Jr. c-Src is required for glial cell line-derived neurotrophic factor (GDNF) family ligand-mediated neuronal survival via a phosphatidylinositol-3 kinase (PI-3K)-dependent pathway. J Neurosci. 2001; 21(5):1464-1472. (Biology: Western blot). View Reference
  3. Lee SW, Bonnah RA, Higashi DL, Atkinson JP, Milgram SL, So M. CD46 is phosphorylated at tyrosine 354 upon infection of epithelial cells by Neisseria gonorrhoeae. J Cell Biol. 2002; 156(6):951-957. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Park J, Cartwright CA. Src activity increases and Yes activity decreases during mitosis of human colon carcinoma cells. Mol Cell Biol. 1995; 15(5):2374-2382. (Biology). View Reference
  5. Sukegawa J, Semba K, Yamanashi Y, et al. Characterization of cDNA clones for the human c-yes gene. Mol Cell Biol. 1987; 7(1):41-47. (Biology). View Reference
View All (5) View Less
610375 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.