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Purified Mouse Anti-XIAP
Purified Mouse Anti-XIAP

Western blot analysis of XIAP on HeLa cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1: 1000 dilution of anti-XIAP/hILP.

Purified Mouse Anti-XIAP

Immunofluorescent staining of MCF7 cells with anti-XIAP/hILP antibody.

Western blot analysis of XIAP on HeLa cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1: 1000 dilution of anti-XIAP/hILP.

Immunofluorescent staining of MCF7 cells with anti-XIAP/hILP antibody.

Product Details
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BD Transduction Laboratories™
hILP
Human (QC Testing), Mouse, Dog (Tested in Development)
Mouse IgG1
Human hILP/XIAP aa. 268-426
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
57 kDa
250 µg/ml
AB_398040
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610717 Rev. 1
Antibody Details
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28/hILP/XIAP

Apoptosis is a genetically programmed, selective process of cell death that occurs during normal cell differentiation and development of multicellular organisms. Viruses depend on the biosynthetic machinery of their host cell for the production of progeny and survival. Therefore, many viruses encode proteins that protect the cell from apoptosis. hILP (human IAP-like protein) is a human homologue of the viral IAP (Inhibitor of Apoptosis Protein). hILP is a widely expressed cytoplasmic protein of 497 amino acids with three BIR (Baculovirus IAP repeats) domains and a C-terminal RING finger domain. hILP-transfected cells are protected against the apoptotic effects of Sindbis virus infection and ICE (interleukin-1β converting enzyme) expression. This product is sold under license from Aegera Therapeutics, Inc.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610717 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610717 Rev.1
Citations & References
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Development References (5)

  1. Duckett CS, Nava VE, Gedrich RW. A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors. EMBO J. 1996; 15(11):2685-2694. (Biology). View Reference
  2. Lee Y, Shacter E. Fas aggregation does not correlate with Fas-mediated apoptosis. J Immunol. 2001; 167(1):82-89. (Clone-specific: Western blot). View Reference
  3. Reffey SB, Wurthner JU, Parks WT, Roberts AB, Duckett CS. X-linked inhibitor of apoptosis protein functions as a cofactor in transforming growth factor-beta signaling. 2001; 276(28):26542-26549. (Clone-specific: Immunofluorescence, Western blot). View Reference
  4. Wang GQ, Gastman BR, Wieckowski E. Apoptosis-resistant mitochondria in T cells selected for resistance to Fas signaling. J Biol Chem. 2001; 276(5):3610-3619. (Clone-specific: Western blot). View Reference
  5. Yang Y, Fang S, Jensen JP, Weissman AM, Ashwell JD. Ubiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science. 2000; 288(5467):874-877. (Clone-specific: Western blot). View Reference
View All (5) View Less
610717 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.