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Purified Mouse Anti-SRPK2
Purified Mouse Anti-SRPK2

Western blot analysis of SRPK2 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-SRPK2 antibody.

Purified Mouse Anti-SRPK2

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Western blot analysis of SRPK2 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-SRPK2 antibody.

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Product Details
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BD Transduction Laboratories™
SR Protein specific Kinase-2
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human SRPK2 aa. 363-485
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
115 kDa
250 µg/ml
AB_398429
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Antibody Details
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23/SRPK2

Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction. However, these interactions are mediated by the non-snRNP factors. The superfamily of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and their function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate RS domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPK1 is predominate in the pancreas, whereas SRPK2 is highly expressed in brain. SRPK2 contains an N-terminal proline-rich sequence that mediates its in vitro interaction with a WW domain containing protein. SRPKs affect splice-site selection and are thought to affect alternative splicing.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (1)

  1. Wang HY, Lin W, Dyck JA. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998; 140(4):737-750. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.