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Purified Mouse Anti-SHP2
Purified Mouse Anti-SHP2

Western blot analysis of SHP2 (PTP1D) on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilultion of the mouse anti- SHP2 (PTP1D) antibody. SHP2 has been reported to be observable in a range between 65-72 kD.

Purified Mouse Anti-SHP2

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of SHP2 (PTP1D) on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilultion of the mouse anti- SHP2 (PTP1D) antibody. SHP2 has been reported to be observable in a range between 65-72 kD.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
PTP1D, Syp, SHPTP2 and PTP2C
Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog (Tested in Development)
Mouse IgG1
Human PTP1D (SHP2) aa. 1-177
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
65-72 kDa
250 µg/ml
AB_397954
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610621 Rev. 1
Antibody Details
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79/PTP1D/SHP2

SHP2 (also known as PTP1D, Syp, SHPTP2 and PTP2C) is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 has been reported to contain two SH2 domains where both SH2 domains are N-terminal to the PTP catalytic domain. The expression of SHP2 has been reported to be highest in brain, heart, and kidney. The PTPs are thought to function with other protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression. The presence of  SH2 domains in SHP2 (PTP1D) has prompted speculation that binding to specific phosphorylated tyrosine residues is key to its function. SHP2 (PTP1D) is tyrosine- phosphorylated and activated in response to stimulation with EGF or PDGF.

610621 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610621 Rev.1
Citations & References
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Development References (5)

  1. Duchene J, Schanstra JP, Pecher C. A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation. J Biol Chem. 2002; 277(43):40375-40383. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Freeman RM, Plutzky J, Neel BG. Identification of a human src homology 2-containing protein-tyrosine-phosphatase: a putative homolog of Drosophila corkscrew. Proc Natl Acad Sci U S A. 1992; 89(23):11239-11243. (Biology). View Reference
  3. Kabat J, Borrego F, Brooks A, Coligan JE. Role that each NKG2A immunoreceptor tyrosine-based inhibitory motif plays in mediating the human CD94/NKG2A inhibitory signal. J Immunol. 2002; 169(4):1948-1958. (Biology: Immunofluorescence). View Reference
  4. Kontaridis MI, Liu X, Zhang L, Bennett AM. Role of SHP-2 in fibroblast growth factor receptor-mediated suppression of myogenesis in C2C12 myoblasts. Mol Cell Biol. 2002; 22(11):3875-3891. (Biology: Immunoprecipitation, Western blot). View Reference
  5. Vogel W, Lammers R, Huang J, Ullrich A. Activation of a phosphotyrosine phosphatase by tyrosine phosphorylation. Science. 1993; 259(5101):1611-1614. (Biology). View Reference
View All (5) View Less
610621 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.