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Purified Mouse Anti-Rab8
Purified Mouse Anti-Rab8

Western blot analysis of Rab8 on a Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Rab8 antibody.

Purified Mouse Anti-Rab8

Immunofluorescence staining of WI-38 cells (human lung fibroblasts; ATCC CCL-75).

Western blot analysis of Rab8 on a Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Rab8 antibody.

Immunofluorescence staining of WI-38 cells (human lung fibroblasts; ATCC CCL-75).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Fly (Tested in Development)
Mouse IgG2b
Human Rab8 aa. 84-205
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
24 kDa
250 µg/ml
AB_398164
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610844 Rev. 1
Antibody Details
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4/Rab8

The members of the Ras superfamily of GTPases, which now includes more than 50 different proteins, shuttle between the GDP-bound inactive position and the GTP-bound active state. The GTPase activity of the Ras-like proteins is regulated by effectors that promote either the GDP- or the GTP-bound states. GTPases of the Ras superfamily have a role in controlling numerous events such as cell proliferation, nucleocytoplasmic transport, and vesicular traffic. The Rab superfamily of proteins includes more than 30 different members that are involved in the regulation of different steps of vesicular traffic. Subcellular localization and specific cellular function of each Rab appears to be encoded in the unique C-terminal part of the proteins, known as the hypervariable region. The 207 amino acid Rab8 protein is closely related to the yeast Sec4p and regulates vesicle transport from the Trans-golgi Network to the basolateral plasma membrane in epithelial cells. In addition, Rab8 interacts with the stress-activated kinase rab8p, a close homologue of the GC Kinase. This interaction was found to be dependent on GTP.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610844 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610844 Rev.1
Citations & References
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Development References (5)

  1. Bahadoran P, Aberdam E, Mantoux F, et al. Rab27a: A key to melanosome transport in human melanocytes. J Cell Biol. 2001; 152(4):843-849. (Biology: Western blot). View Reference
  2. Chavrier P, Vingron M, Sander C, Simons K, Zerial M. Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell line. Mol Cell Biol. 1990; 10(12):6578-6585. (Biology). View Reference
  3. Hume AN, Collinson LM, Rapak A, Gomes AQ, Hopkins CR, Seabra MC. Rab27a regulates the peripheral distribution of melanosomes in melanocytes. J Cell Biol. 2001; 152(4):795-808. (Biology: Western blot). View Reference
  4. Kessels MM, Engqvist-Goldstein AE, Drubin DG. Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation. Mol Biol Cell. 2000; 11(1):393-412. (Biology: Immunofluorescence). View Reference
  5. Nimmo ER, Sanders PG, Padua RA, Hughes D, Williamson R, Johnson KJ. The MEL gene: a new member of the RAB/YPT class of RAS-related genes. Oncogene. 1991; 6(8):1347-1351. (Biology). View Reference
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610844 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.