Skip to main content Skip to navigation
Purified Mouse Anti-PTP1C/SHP1
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human PTP1C aa.. 492-597
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
68 kDa
250 µg/ml
AB_397532
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610125 Rev. 1
Antibody Details
Down Arrow Up Arrow
52/PTP1C/SHP1

PTP1C (SH-PTP1, SHP-1, or HCP) is a protein-tyrosine phosphatase (PTP) that is expressed in epithelial and hematopoietic cells. It contains two N-terminal SH2 domains and a C-terminal phosphatase domain. While its distribution is primarily cytosolic, exposure to various stimuli induces movement of PTP1C to other cellular locations to allow interaction with its substrates. This enzyme associates with the IL-3, IL-4, erythropoietin, and stem cell factor receptors and may regulate their signaling pathways by dephosphorylation of their receptors or their downstream effectors. Motheaten mice, which possess a nonfunctional PTP1C gene, display multiple hematopoietic abnormalities including increased proliferation and activation of myeloid cells. Studies involving these mice have clearly implicated PTP1C in negative regulation of CSF-1 (colony stimulating factor-1) mitogenic signaling and have demonstrated a role for PTP1C as an intermediate between Ras and the MAP kinase pathway.

610125 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610125 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Duchene J, Schanstra JP, Pecher C. A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation. J Biol Chem. 2002; 277(43):40375-40383. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  2. Keilhack H, Muller M, Bohmer SA, et al. Negative regulation of Ros receptor tyrosine kinase signaling. An epithelial function of the SH2 domain protein tyrosine phosphatase SHP-1. J Cell Biol. 2001; 152(2):325-334. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  3. Kenner KA, Anyanwu E, Olefsky JM, Kusari J. Protein-tyrosine phosphatase 1B is a negative regulator of insulin- and insulin-like growth factor-I-stimulated signaling. J Biol Chem. 1996; 271(33):19810-19816. (Biology). View Reference
  4. Liu F, Hill DE, Chernoff J. Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas). J Biol Chem. 1996; 271(49):31290-31295. (Biology). View Reference
  5. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Clone-specific: Flow cytometry, Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610125 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

23-22940-00