Skip to main content Skip to navigation
Purified Mouse Anti-PNUTS
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Rat PNUTS aa. 605-716
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
110 kDa
250 µg/ml
AB_398373
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611060 Rev. 1
Antibody Details
Down Arrow Up Arrow
47/PNUTS

Many aspects of cellular physiology are influenced by the serine/threonine protein phosphatase1 (PP1). It is a highly conserved eukaryotic protein that reverses the action of protein kinases. PP1 is key to such processes as cell division, neuronal metabolism, and protein synthesis. It consists of a catalytic subunit, with a broad substrate specificity, whose action is directed by its association with a family of regulatory proteins including inhibitor-1, inhibitor-2, and NIPP-1. In response to extracellular stimuli, these proteins interact with PP1 and inhibit its catalytic activity. Other regulatory proteins are referred to as targeting subunits because they direct PP1 to specific subcellular locations and modulate its activity. In the cell nucleus, PP1 has been implicated in a variety of processes. Nuclear PP1 exists in high MW complexes with other proteins including NIPP-1. PNUTS (phosphatase 1 nuclear targeting subunit) is a ubiquitously expressed nuclear protein that forms a stable complex with PP1 and is thought to modulate its catalytic activity. Thus, PNUTS is a specific regulatory protein that directs the nuclear function of PP1.

611060 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611060 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (1)

  1. Allen PB, Kwon YG, Nairn AC, Greengard P. Isolation and characterization of PNUTS, a putative protein phosphatase 1 nuclear targeting subunit. J Biol Chem. 1998; 273(7):4089-4095. (Biology). View Reference
611060 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.