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Purified Mouse Anti-p38α
Product Details
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BD Transduction Laboratories™
SAPK2a
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human p38 MAPK aa. 243-355
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
42 kDa
250 µg/ml
AB_399540
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612169 Rev. 2
Antibody Details
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27/p38α/SAPK2a

Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-α. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). One of these, p38 MAP kinase (p38 MAPK or CSPB2), is a human homologue of HOG1, a yeast MAP kinase that is essential for growth under conditions of elevated osmolarity. Similar to HOG1, p38 MAPK is phosphorylated in response to hyperosmolarity, but also in response to a variety of stimuli including LPS, IL-1, or TNF-α. Efficient activation of p38 MAPK requires phosphorylation of Thr-180 and Tyr-182. At least three Thr/Tyr kinases (MKK3, MKK4/SEK1, and MKK6) phosphorylate and activate p38 MAPK. This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP/GADD143) that induce expression of proinflammatory cytokine genes. In addition to its role in immunity, p38 MAPK is widely expressed and serves as an important signal transducer in a variety of cell types.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612169 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612169 Rev.2
Citations & References
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Development References (4)

  1. Brunet A, Pouyssegur J. Identification of MAP kinase domains by redirecting stress signals into growth factor responses. Science. 1996; 272(5268):1652-1655. (Biology). View Reference
  2. Han J, Lee JD, Bibbs L, Ulevitch RJ. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science. 1994; 265(5173):808-811. (Biology). View Reference
  3. Morinobu A, Gadina M, Strober W, et al. STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation. Proc Natl Acad Sci U S A. 2002; 99(19):12281-12286. (Biology: Western blot). View Reference
  4. Winston BW, Chan ED, Johnson GL, Riches DW. Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages. J Immunol. 1997; 159(9):4491-4497. (Biology). View Reference
View All (4) View Less
612169 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.