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Purified Mouse Anti-p120 Catenin
Purified Mouse Anti-p120 Catenin
Western blot analysis of p120 Catenin on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-p120 Catenin antibody.
Purified Mouse Anti-p120 Catenin
Immunofluorescence staining of human fibroblasts.
Western blot analysis of p120 Catenin on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-p120 Catenin antibody.
Immunofluorescence staining of human fibroblasts.
Product Details
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BD Transduction Laboratories™
pp120 (Src Substrate); p120cas
Human (QC Testing), Mouse,Rat,Chicken,Dog (Tested in Development)
Mouse IgG1
Mouse pp120 aa. 326-632
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
120 kDa
250 µg/ml
AB_397537
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610133 Rev. 1
Antibody Details
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98/pp120

The membrane associated protein p120 Catenin (pp120 (Src substrate); p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation.  It shares structural similarity with the Drosophila Armadillo protein and the vertebrate β-catenin and γ-catenin proteins.  This similarity is evidenced by its characteristic Arm domain that is composed of 42-amino acid motif repeats.  In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex.  Like β- and γ-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins.  It exists as four isoforms that range in size from 90-115 kDa.  Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions.  Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation.  Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.

610133 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610133 Rev.1
Citations & References
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Development References (5)

  1. Eger A, Stockinger A, Schaffhauser B, Beug H, Foisner R. Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity. J Cell Biol. 2000; 148(1):173-187. (Biology: Electron microscopy, Western blot). View Reference
  2. Lampugnani MG, Zanetti A, Breviario F, et al. VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam. Mol Biol Cell. 2002; 13(4):1175-1189. (Biology: Immunofluorescence, Western blot). View Reference
  3. Laura RP, Witt AS, Held HA. The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF. J Biol Chem. 2002; 277(15):12906-12914. (Biology: Western blot). View Reference
  4. Noren NK, Liu BP, Burridge K, Kreft B. p120 catenin regulates the actin cytoskeleton via Rho family GTPases. J Cell Biol. 2000; 150(3):567-579. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  5. Wine RN, Chapin RE. Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat. J Androl. 1999; 20(2):198-213. (Biology: Immunohistochemistry, Western blot). View Reference
View All (5) View Less
610133 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.