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Western blot analysis of P-Cadherin on A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-P-Cadherin antibody.
Immunofluorescent staining of A431 cells.
BD Transduction Laboratories™ Purified Mouse Anti-P-Cadherin
BD Transduction Laboratories™ Purified Mouse Anti-P-Cadherin
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Cadherins are similar in their domain structure, Ca2+ and protease sensitivity, and molecular weight. However, these proteins have distinct tissue expression patterns and immunological reactivities. P-Cadherin is localized in placenta while E-Cadherin and N-Cadherin are found in epithelial and neural tissues, respectively. P-Cadherin is an 829 amino acid polypeptide with a putative signal peptide and precursor region, an extracellular domain containing several internal repeats, and a highly hydrophobic transmembrane region. The cytoplasmic domain provides a link to the cytoskeleton through the associated catenin proteins.
Development References (5)
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Bailey T, Biddlestone L, Shepherd N, Barr H, Warner P, Jankowski J. Altered cadherin and catenin complexes in the Barrett's esophagus-dysplasia-adenocarcinoma sequence: correlation with disease progression and dedifferentiation. Am J Pathol. 1998; 152(1):135-144. (Clone-specific: Immunohistochemistry). View Reference
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Holsinger LJ, Ward K, Duffield B, Zachwieja J, Jallal B. The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn). Oncogene. 2002; 21(46):7067-7076. (Clone-specific: Western blot). View Reference
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Kantak SS, Kramer RH. E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells. J Biol Chem. 1998; 273(27):16953-16961. (Clone-specific: Western blot). View Reference
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Nose A, Nagafuchi A, Takeichi M. Isolation of placental cadherin cDNA: identification of a novel gene family of cell-cell adhesion molecules. EMBO J. 1987; 6(12):3655-3661. (Biology). View Reference
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Tao YS, Edwards RA, Tubb B, Wang S, Bryan J, McCrea PD. beta-Catenin associates with the actin-bundling protein fascin in a noncadherin complex. J Cell Biol. 1996; 134(5):1271-1281. (Clone-specific: Immunofluorescence). View Reference
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