Purified Mouse Anti-Neuronal Pentraxin

BD Transduction Laboratories™ Purified Mouse Anti-Neuronal Pentraxin

Name: Purified Mouse Anti-Neuronal Pentraxin
Brand: BD Transduction Laboratories™
SKU: 610369

Clone 22/Neuronal Pentraxin

(RUO)

Western blot analysis of Neuronal Pentraxin on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of Neuronal Pentraxin.

Rat Brain

Western blot analysis of Neuronal Pentraxin on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of Neuronal Pentraxin.

Rat Brain

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Scientific Resources

Product Details

Brand: BD Transduction Laboratories™

Reactivity: Rat (QC Testing), Human (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Rat Neuronal Pentraxin aa. 137-312

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)

Target Molecular Weight: 47 kDa

Concentration: 250 µg/ml

RRID: AB_397754

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

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Antibody Details

22/Neuronal Pentraxin

Neuronal pentraxin (NP) is a potential receptor for the snake venom toxin, taipoxin. The highest levels of NP mRNA are found in the cerebellum and the CA3 region of the hippocampus. More specifically, NP mRNA is expressed at high levels in Purkinje and granule neurons of the cerebellum and CA3 neurons of the hippocampus. Moderate levels are present in neurons of the hilus, dentate gyrus, CA1, cerebral cortex, medial habenula, anterior hypothalamic nuclei, olfactory cortex, and inferior olivary nuclei. NP mRNA is not expressed in glia of white or gray matter. It is thought that NP serves as a receptor for taipoxin and, after binding, NP-taipoxin is taken into presynaptic terminals. Alternatively, the binding of taipoxin by NP may represent a nonspecific mechanism to sequester toxins from synapses.

610369 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

610369 Rev.1

Citations & References

Development References (2)

DeGregorio-Rocasolano N, Gasull T, Trullas R. Overexpression of neuronal pentraxin 1 is involved in neuronal death evoked by low K(+) in cerebellar granule cells. Neuron. 2001; 276(1):796-803. (Clone-specific: Western blot). View Reference
Schlimgen AK, Helms JA, Vogel H, Perin MS. Neuronal pentraxin, a secreted protein with homology to acute phase proteins of the immune system. Neuron. 1995; 14(3):519-526. (Biology). View Reference

610369 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.