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Purified Mouse Anti- Munc-18
Purified Mouse Anti- Munc-18

Western blot analysis of Munc-18 on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti- Munc-18 antibody.

Purified Mouse Anti- Munc-18

Immunohistochemical staining for Munc-18 on a formalin-fixed, paraffin-embedded rat brain section with citrate buffer pretreatment (40X magnification).

Western blot analysis of Munc-18 on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti- Munc-18 antibody.

Immunohistochemical staining for Munc-18 on a formalin-fixed, paraffin-embedded rat brain section with citrate buffer pretreatment (40X magnification).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Rat Munc-18-1 aa. 381-567
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
68 kDa
250 µg/ml
AB_397727
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610337 Rev. 1
Antibody Details
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31/Munc-18

There are two Munc-18 isoforms: Munc-18-1 and Munc-18-2. Munc-18-1 (n-sec1 or rb-sec1; 68 kDa) is a neuronal protein that binds tightly to syntaxin 1 and functions in synaptic vesicle exocytosis. Munc-18-2 exhibits 63% amino acid sequence identity with Munc-18-1. Although Munc-18-2 is expressed in most tissues, Munc-18-1 is primarily expressed in brain. Both forms of Munc-18 bind tightly to syntaxins 1A, 2, and 3, but not to syntaxin 4. Syntaxin 1A is a plasma membrane protein implicated in synaptic vesicle docking. Following calcium entry into the presynaptic nerve terminal, the neurotransmitter-containing synaptic vesicle fuses with the plasma membrane at a region known as the active zone. Thus, by virtue of its interaction with syntaxin 1A, Munc-18-1 is thought to have an essential function in neurotransmitter release.

610337 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610337 Rev.1
Citations & References
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Development References (5)

  1. Fisher RJ, Pevsner J, Burgoyne RD. Control of fusion pore dynamics during exocytosis by Munc18. Science. 2001; 291(5505):875-878. (Biology: Immunofluorescence). View Reference
  2. Hata Y, Slaughter CA, Sudhof TC. Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin. Nature. 1993; 366(6453):347-351. (Biology). View Reference
  3. Shuang R, Zhang L, Fletcher A, Groblewski GE, Pevsner J, Stuenkel EL. Regulation of Munc-18/syntaxin 1A interaction by cyclin-dependent kinase 5 in nerve endings. J Biol Chem. 1998; 273(9):4957-4966. (Biology: Immunoprecipitation, Western blot). View Reference
  4. Torii S, Zhao S, Yi Z, Takeuchi T, Izumi T. Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a. Mol Cell Biol. 2002; 22(15):5518-5526. (Biology: Western blot). View Reference
  5. Zhang W, Efanov A, Yang SN, et al. Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell. J Biol Chem. 2000; 275(52):41521-41527. (Biology: Western blot). View Reference
View All (5) View Less
610337 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.