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Western blot analysis of MAD2B on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- MAD2B antibody.
Immunofluorescence staining of human endothelial cells.
BD Transduction Laboratories™ Purified Mouse Anti-MAD2B
BD Transduction Laboratories™ Purified Mouse Anti-MAD2B
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase ζ subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (2)
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Chen J, Fang G. MAD2B is an inhibitor of the anaphase-promoting complex. Genes Dev. 2000; 15(14):1765-1770. (Biology). View Reference
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Murakumo Y, Roth T, Ishii H, et al. A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2. J Biol Chem. 2000; 275(6):4391-4397. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.