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Purified Mouse Anti-MAD2B
Purified Mouse Anti-MAD2B

Western blot analysis of MAD2B on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- MAD2B antibody.

Purified Mouse Anti-MAD2B

Immunofluorescence staining of human endothelial cells.

Western blot analysis of MAD2B on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- MAD2B antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Mitotic Arrest Deficient-2B; Rev7
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human MAD2B aa. 81-180
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
26 kDa
250 µg/ml
AB_399583
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612266 Rev. 1
Antibody Details
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14/MAD2B/Rev7

Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC.  Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase ζ subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612266 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612266 Rev.1
Citations & References
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Development References (2)

  1. Chen J, Fang G. MAD2B is an inhibitor of the anaphase-promoting complex. Genes Dev. 2000; 15(14):1765-1770. (Biology). View Reference
  2. Murakumo Y, Roth T, Ishii H, et al. A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2. J Biol Chem. 2000; 275(6):4391-4397. (Biology). View Reference
612266 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.