BD Transduction Laboratories™ Purified Mouse Anti-LSF

LSF (αCP2 or LBP-1c) is a transcription factor originally identified by its binding to, and induction of, the SV40 late promoter. LSF displays some similarity to the Elf-1/NTF transcription factors of Drosophila. It is primarily found in its dimeric form in solution, however it is the tetrameric form that recognizes a center-to-center 10 bp repeat. LSF-1D (LBP-1d) is the product of an alternative splicing event, resulting in a deletion of a 51 amino acid segment that corresponds with the DNA-binding region of LSF. LSF-1D is found in the cytoplasm, not in the nucleus, and can interact with LSF to form a heterotetramer that can interfere with LSF DNA binding. In mitogen-stimulated human peripheral T cells, LSF is  phosphorylated on amino acid 291, which increases its DNA binding activity. Thus, LSF-mediated gene transcription is regulated by its phosphorylation and/or its interaction with LSF-1D during cell growth.

This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.