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Purified Mouse Anti-LAP2
Purified Mouse Anti-LAP2

Western blot analysis of LAP2 on a RSV-3T3 cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-LAP2 antibody.

Purified Mouse Anti-LAP2

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of LAP2 on a RSV-3T3 cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-LAP2 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
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BD Transduction Laboratories™
LAP2β; Lamina-Associated Polypeptides
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Rat LAP2 aa. 34-156
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
53 kDa
250 µg/ml
AB_398313
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611000 Rev. 1
Antibody Details
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27/LAP2

A specialized extension of the ER, the nuclear envelope (NE) forms the nuclear compartment boundary in eukaryotic cells. It contains numerous pore complexes and the nucleoplasmic side is linked to nuclear lamina. The nuclear lamina composes the structural framework for the NE and serves as a chromatin anchor site, thus, playing a major role in interphase nuclear organization. Many proteins are associated with lamina, particularly the LAPs (Lamina-Associated Polypeptides). LAP2 (also known as LAP2β) is a hydrophilic protein with a single transmembrane segment near the C-terminus. Thus, it has been defined as a type II integral membrane protein with the majority of its structure exposed to the nucleoplasm. LAP2 binding to lamins contributes to the attachment of the nuclear lamina to the inner nuclear membrane. LAP2 also binds to chromatin, implying its role in chromosomal organization during mitosis. Mitotic phosphorylation of LAP2 regulates its binding to lamins and chromosomes during the disassembly and reassembly of mitosis. Thus, LAP2 is a nuclear protein that plays a role in the organization of the NE during cell cycle progression.

611000 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611000 Rev.1
Citations & References
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Development References (5)

  1. Dechat T, Gotzmann J, Stockinger A, et al. Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics. EMBO J. 1998; 17(16):4887-4902. (Biology). View Reference
  2. Furukawa K, Fritze CE, Gerace L. The major nuclear envelope targeting domain of LAP2 coincides with its lamin binding region but is distinct from its chromatin interaction domain. J Biol Chem. 1998; 273(7):4213-4219. (Biology). View Reference
  3. Furukawa K, Pante N, Aebi U, Gerace L. Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope. EMBO J. 1995; 14(8):1626-1636. (Biology). View Reference
  4. Kimura T, Ito C, Watanabe S, et al. Mouse germ cell-less as an essential component for nuclear integrity. Mol Cell Biol. 2003; 23(4):1304-1315. (Biology: Immunofluorescence). View Reference
  5. Rusan NM, Tulu US, Fagerstrom C, Wadsworth P. Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport. J Biol Chem. 2002; 158(6):997-1003. (Biology: Immunofluorescence). View Reference
View All (5) View Less
611000 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.