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Purified Mouse Anti-IKKγ
Purified Mouse Anti-IKKγ
Western blot analysis of IKKγ on rat kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-IKKγ antibody.
Purified Mouse Anti-IKKγ
Immunofluorescent staining of ES2 cells with anti-IKKγ antibody.
Western blot analysis of IKKγ on rat kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-IKKγ antibody.
Immunofluorescent staining of ES2 cells with anti-IKKγ antibody.
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human,Dog (Tested in Development)
Mouse IgG1
Mouse IKKγ/NEMO aa. 278-396
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
48 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611306 Rev. 1
Antibody Details
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In most cells, NF-κB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IκBα, IκBβ, and IκBε. Cell stimulation induces the release, activation, and nuclear translocation of NF-κB. Release of NF-κB results from the phosphorylation and subsequent proteolytic degradation of the IκB proteins. Two cytokine-inducible IκB kinases (IKKα and IKKβ) phosphorylate and target the IκB proteins for degradation by the ubiquitin pathway. These kinases are components of a 700-900 kDa multisubunit complex that also contains NF-κB/RelA, IκBα, MEKK1, NIK, IKAP, and IKKγ/NEMO (NF-κB essential modulator). IKKγ contains two coiled-coil domains and a leucine zipper which allow it to form dimers and trimers that interact directly with IKKβ. IKKγ, essential for IKKα/IKKβ activation of NF-κB, is located functionally and physically upstream of these subunits. In addition, IKKγ enables the HTLV-1 Tax oncoprotein to interact with IKKβ, resulting in constitutive activation of NF-κB and maintenance of cellular transformation. Thus, IKKγ is an essential element of the IκB complex and an adaptor that mediates stable formation of Tax-IKK complexes.

611306 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611306 Rev.1
Citations & References
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View product citations for antibody "611306" on CiteAb

Development References (5)

  1. Chu ZL, Shin YA, Yang JM, DiDonato JA, Ballard DW. IKKgamma mediates the interaction of cellular IkappaB kinases with the tax transforming protein of human T cell leukemia virus type 1. J Biol Chem. 1999; 274(22):15297-15300. (Biology). View Reference
  2. Jin DY, Giordano V, Kibler KV, Nakano H, Jeang KT. Role of adapter function in oncoprotein-mediated activation of NF-kappaB. Human T-cell leukemia virus type I Tax interacts directly with IkappaB kinase gamma. J Biol Chem. 1999; 274(25):17402-17405. (Biology). View Reference
  3. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Biology). View Reference
  4. Weil R, Schwamborn K, Alcover A, Bessia C, Di Bartolo V, Israël A. Induction of the NF-kappaB cascade by recruitment of the scaffold molecule NEMO to the T cell receptor. Immunity. 2003; 18(1):13-26. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  5. Yamaoka S, Courtois G, Bessia C. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation. Cell. 1998; 93(7):1231-1240. (Biology). View Reference
View All (5) View Less
611306 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.