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Purified Mouse Anti-Human Nup88
Purified Mouse Anti-Human Nup88

Western blot analysis of Nup88 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human Nup88 antibody.

Purified Mouse Anti-Human Nup88

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of Nup88 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human Nup88 antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Nup88 aa. 314-425
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
88 kDa
250 µg/ml
AB_399376
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611896 Rev. 2
Antibody Details
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22/Nup88

Nucleocytoplasmic transport involves the binding and interaction of several cytosolic and nuclear pore proteins by at least two different mechanisms. One mechanism is mediated by karyopherins to transport proteins with the nuclear localization sequence (NLS) and an alternative pathway uses the protein Transportin. Morphologically both pathways require the use of the nuclear pore complex (NPC) which acts as a gate mediating active transport of proteins and RNA into and out of the nucleus. The NLS binds to karyopherin α, and to the N-terminal region of karyopherin β. Both karyopherins bind to repeat sequences of nucleoporins at the nuclear envelope. p62 is the best characterized member of a group of nucleoporins that line the central region of the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58.  Nup88 is a nonglycosylated nucleoporin, that does not contain the characteristic GLFG or XFXFG repeats found in other NPC proteins. The structure of Nup88 includes a coiled-coil domain required for association with the NPC. Nup88 is overexpressed during oncogenesis and development, and forms a complex with the nuclear transport factors, Exportin-1 and Nup214.

611896 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611896 Rev.2
Citations & References
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Development References (3)

  1. Bastos R, Ribas de Pouplana L, Enarson M, Bodoor K, Burke B. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. J Cell Biol. 1997; 137(5):989-1000. (Biology). View Reference
  2. Fornerod M, van Deursen J, van Baal S. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J. 1997; 16(4):807-816. (Biology). View Reference
  3. Martinez N, Alonso A, Moragues MD, Ponton J, Schneider J. The nuclear pore complex protein Nup88 is overexpressed in tumor cells. Cancer Res. 1999; 59(21):5408-5411. (Biology). View Reference
611896 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.