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Purified Mouse Anti-Human MSH3
Purified Mouse Anti-Human MSH3

Western blot analysis of MSH3 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). 2 µg/mL (lane 1), 1 µg/mL (lane 2) and 0.5 µg/mL (lane 3) of the mouse anti-human MSH3 antibody were used.

Purified Mouse Anti-Human MSH3

Immunofluorescence staining of human fibroblasts.

Western blot analysis of MSH3 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). 2 µg/mL (lane 1), 1 µg/mL (lane 2) and 0.5 µg/mL (lane 3) of the mouse anti-human MSH3 antibody were used.

Immunofluorescence staining of human fibroblasts.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human MSH3 aa. 136-349
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
127 kDa
250 µg/ml
AB_398912
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611390 Rev. 2
Antibody Details
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52/MSH3

Bacterial mismatch DNA repair involves the MutL, MutH, and MutS proteins, which forms a complex that mediates excision repair. Mutations in or deficiencies of any of these proteins results in a mutator phenotype that is characterized by genetic instability. Human homologs of MutS include MSH2, MSH3, and MSH6. MSH2 forms heterodimers with MSH6 (hMutSα) or MSH3 (hMutSβ) that specifically bind single-mispaired nucleotides and a subset of insertion-deletion mismatches. In addition, these heterodimers have intrinsic ATPase activity that is regulated by mismatch binding. ADP-bound heterodimers bind mismatched nucleotides, while ATP-bound heterodimers do not. The role of MSH3 in genetic stability in human cells in unclear. However, MSH3 and MSH6 share roles in the control of mutation rates. Both participate in repair of replication errors containing base-base mismatches or 1-4 extra bases. The MSH3 gene is located upstream of the dihydrofolate reductase (DHFR) gene and is expressed at low levels in a variety of human tissues. Thus, MSH3 is a component of an adenosine nucleotide-regulated molecular switch whose activity is essential for classical nucleotide mismatch repair.

611390 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611390 Rev.2
Citations & References
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Development References (4)

  1. New L, Liu K, Crouse GF. The yeast gene MSH3 defines a new class of eukaryotic MutS homologues. Mol Gen Genet. 1993; 239(1-2):97-108. (Biology). View Reference
  2. Umar A, Risinger JI, Glaab WE, Tindall KR, Barrett JC, Kunkel TA. Functional overlap in mismatch repair by human MSH3 and MSH6. Genetics. 1998; 148(4):1637-1646. (Biology). View Reference
  3. Watanabe A, Ikejima M, Suzuki N, Shimada T. Genomic organization and expression of the human MSH3 gene. Genomics. 1996; 31(3):311-318. (Biology). View Reference
  4. Wilson T, Guerrette S, Fishel R. Dissociation of mismatch recognition and ATPase activity by hMSH2-hMSH3. J Biol Chem. 1999; 274(31):21659-21664. (Biology). View Reference
View All (4) View Less
611390 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.