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Purified Mouse Anti-Human IGFBP-3
Purified Mouse Anti-Human IGFBP-3

Western blot analysis of IGFBP-3 on a human plasma lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human IGFBP-3 antibody.

Purified Mouse Anti-Human IGFBP-3

Immunofluorescence staining of human fibroblasts.

Western blot analysis of IGFBP-3 on a human plasma lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human IGFBP-3 antibody.

Immunofluorescence staining of human fibroblasts.

Product Details
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BD Transduction Laboratories™
IGF-Binding Protein-3
Human (QC Testing)
Mouse IgG1
Human IGFBP-3 aa. 101-210
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
40 & 44 kDa
250 µg/ml
AB_398964
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611504 Rev. 1
Antibody Details
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4/IGFBP-3

Insulin-like growth factors (IGF) I and II are peptide hormones that regulate cellular proliferation and differentiation.  Most circulating IGFs exist in 130-150 kDa ternary complexes containing IGF-Binding Protein-3 (IGFBP-3) and an 85 kDa glycoprotein, the acid-labile subunit (ALS).  IGFBP-3 is one of six IGFBPs that, by binding IGF peptides, prolong their half life and maintain the IGF reservoir.  IGFBP-3 is found in body fluids as multiple 40-50 kDa forms as a result of differential glycosylation.  The expression of IGFBP-3 in many tissues suggests that it locally modulates the autocrine/paracrine action of IGF peptides.  IGFBP-3 binding to fibrinogen may be important for wound healing, since this concentrates IGF-I at wound sites and lowers the affinity of IGF-I for IGFBP-3.  In addition, retinoic acid-induced IGFBP-3 expression inhibits the growth promoting effects of IGF-I in breast cancer cells.  This may link the retinoid and IGF systems in cell growth regulation and explain how the loss of retinoic acid receptor β leads to breast cancer progression.  Thus, IGFBP-3 maintenance and regulation of IGF activity in various tissues may have diverse physiological roles.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (4)

  1. Campbell PG, Durham SK, Hayes JD, Suwanichkul A, Powell DR. Insulin-like growth factor-binding protein-3 binds fibrinogen and fibrin. J Biol Chem. 1999; 274(42):30215-30221. (Biology). View Reference
  2. Cubbage ML, Suwanichkul A, Powell DR. Insulin-like growth factor binding protein-3. Organization of the human chromosomal gene and demonstration of promoter activity. J Biol Chem. 1999; 265(21):12642-12649. (Biology). View Reference
  3. Janosi JB, Firth SM, Bond JJ, Baxter RC, Delhanty PJ. N-Linked glycosylation and sialylation of the acid-labile subunit. Role in complex formation with insulin-like growth factor (IGF)-binding protein-3 and the IGFs. J Biol Chem. 1999; 274(9):5292-5298. (Biology). View Reference
  4. Shang Y, Baumrucker CR, Green MH. Signal relay by retinoic acid receptors alpha and beta in the retinoic acid-induced expression of insulin-like growth factor-binding protein-3 in breast cancer cells. J Biol Chem. 1999; 274(25):18005-18010. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.