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Purified Mouse Anti-Human EFP
Purified Mouse Anti-Human EFP

Western blot analysis of EFP on a human endothelial cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-human EFP antibody.

Purified Mouse Anti-Human EFP

Immunofluorescence staining of human endothelial cells.

Western blot analysis of EFP on a human endothelial cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-human EFP antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Estrogen receptor Finger Protein
Human (QC Testing)
Mouse IgG1
Human EFP aa. 32-210
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
71 kDa
250 µg/ml
AB_397919
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610570 Rev. 1
Antibody Details
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2/EFP

Steriod hormones, unlike polypeptide hormones, diffuse inside the cell, bind cytoplasmic or nuclear receptors, and activate specific genes.  Complete identification of such receptors would greatly increase the understanding of the celluar processes activated by steriods.  EFP (Estrogen receptor Finger Protein) was identified as a result of its specific interaction with the estrogen receptor.  EFP contains a zinc-finger domain, indicating that it is a DNA-binding protein.  EFP transcription is regulated by estrogens and, in combination with the estrogen receptor, it may modulate the expression of estrogen-responsive genes.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610570 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610570 Rev.1
Citations & References
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Development References (1)

  1. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.. Proc Natl Acad Sci U S A. 1993 ; 90((23):11117-11121. (Biology). View Reference
610570 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.