Skip to main content Skip to navigation
Purified Mouse Anti-Human Cip1
Purified Mouse Anti-Human Cip1

Western blot analysis of Cip1 on a WI-38 cell lysate (Human lung fibroblasts; ATCC CCL-75).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human Cip1 antibody.

Purified Mouse Anti-Human Cip1

Immunofluorescence staining of human fibroblasts.

Western blot analysis of Cip1 on a WI-38 cell lysate (Human lung fibroblasts; ATCC CCL-75).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human Cip1 antibody.

Immunofluorescence staining of human fibroblasts.

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
WAF1
Human (QC Testing)
Mouse IgG2a
Human Cip1 aa. 1-150
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
21 kDa
250 µg/ml
AB_397629
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610233 Rev. 1
Antibody Details
Down Arrow Up Arrow
70/Cip1/WAF1

Cip1 (p21) was identified as an inhibitor of cdk activity in a quaternary complex that also included Cyclin D, Cdk4, and PCNA. It has subsequently been shown that Cip1 can directly bind to and inhibit each member of the cdk family, though the affinities vary for each enzyme. Several studies indicate that Cip1 expression is regulated by the p53 tumor suppressor protein. For example, following DNA damage, Cip1 becomes transcriptionally induced in a p53-dependent manner. Thus, Cip1 protein may have a prominent role in mediating cell cycle arrest. Cip1 is also a component of active cyclin/cdk kinases. It has been suggested that Cip1-containing enzymes may transition between active and inactive states through changes in Cip1 stoichiometry. Active complexes appear to contain a single Cip1 molecule, while the inactive complexes have multiple Cip1 subunits. When multiple subunits are complexed with a cdk, cyclin, and PCNA, these Cip1 molecules can block the access of cdk-activating kinase (CAK) to cdk, thus preventing its phosphorylation and activation. However, inhibition of cdk activity by Cip1 does not appear to be dependent upon this mechanism. Other studies on DNA replication indicate that Cip1 can inhibit this process in vitro by directly binding to PCNA, a DNA polymerase-δ processivity factor.

610233 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610233 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Carrano AC, Pagano M. Role of the F-box protein Skp2 in adhesion-dependent cell cycle progression. J Cell Biol. 2001; 153(7):1381-1389. (Biology: Western blot). View Reference
  2. Cheng L, Lloyd RV, Weaver AL. The cell cycle inhibitors p21WAF1 and p27KIP1 are associated with survival in patients treated by salvage prostatectomy after radiation therapy. Clin Cancer Res. 2000; 6(5):1896-1899. (Biology: Immunohistochemistry). View Reference
  3. Dulic V, Stein GH, Far DF, Reed SI. Nuclear accumulation of p21Cip1 at the onset of mitosis: a role at the G2/M-phase transition. Mol Cell Biol. 1998; 18(1):546-557. (Biology: Immunofluorescence, Western blot). View Reference
  4. Fima E, Shtutman M, Libros P. PKCeta enhances cell cycle progression, the expression of G1 cyclins and p21 in MCF-7 cells. Oncogene. 2001; 20(46):6794-6804. (Biology: Immunoprecipitation, Western blot). View Reference
  5. Yan Z, Deng X, Friedman E. Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III. J Biol Chem. 2001; 276(2):1555-1563. (Biology: Western blot). View Reference
View All (5) View Less
610233 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.